《植物生理学报》 2013, 49(11): 1168-1172
通信作者:蓝兴国;E-mail: lanxingguo@126.com;Tel: 0451-82191783
摘 要:
摘要: 单克隆抗体是现代生命科学研究的重要工具。随着分子生物学的发展, 单克隆抗体在植物研究中发挥着越来越重要的作用。本文综述了单克隆抗体在蛋白表达、蛋白定位、蛋白相互作用、植物成分的定性与定量、植物成分纯化、植物病害检测、标签抗体等方面研究中的应用。关键词:植物; 单克隆抗体; 蛋白表达; 蛋白定位; 蛋白质相互作用; 标签抗体
收稿:2013-06-17 修定:2013-08-22
资助:中央高校基本科研业务费专项基金(DL13CA13)和国家自然科学基金(30900115和31070275)
Corresponding author: LAN Xing-Guo; E-mail: lanxingguo@126.com; Tel: 0451-82191783
Abstract:
Monoclonal antibody is an important tool in modern life science. With major developments in molecular biology, monoclonal antibody has wide application in plants. Here, the monoclonal antibody’s application in protein expression, protein localization, protein-protein interaction, determine the nature and ration of plant component, purification of plant component, detection of Plant disease and tag antibody are introduced.Key words: plant; monoclonal antibody technique; protein expression; protein localization; protein-protein interaction; tag antibody
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